Our project focuses on bone cell interactions that are implicated in bone pathologies. Based on our previous work, we study bone cells and their communications through soluble mediators that appeared to play an important role in bone healing, inflammatory arthritis and primary bone tumors. Our project aims:

  • To characterize the interactions between stromal cells (mesenchymal stem cells, fibroblasts, osteoblasts) and myeloid cells (monocytes, macrophages, osteoclasts) in bone healing or inflammatory arthritis.
  • To model cell interactions in primary bone tumors by using co-cultures and 3D cultures.
  • To propose new therapies for bone pathologies including primary bone tumors based on these cell interactions.
Our team gathers 2 researchers with complementary expertise (F. Blanchard and V. Trichet) and clinicians from the academic hospital of Nantes and Tours (B. Le Goff, rheumatologist; P. Perrot, plastic surgeon; C. Nich and LR. Lenail, orthopedic surgeons).

Axis 1. Myeloid and stromal cells in chronic inflammation.

PI: Frédéric Blanchard and Benoit Le Goff.

In vitro and in vivo study of communications between myeloid (macrophages, osteoclasts) and stromal (synovial fibroblasts, osteoblasts) cells: role and mechanisms of action of novel cytokines from IL-1 family that are produced by macrophages in rheumatoid arthritis.

More details here.

Axis 2. Stromal cells in the bone tumor niche.

PI: Valérie Trichet and Louis Romée Lenail.

In vitro and in vivo study of stromal cells in bone sarcomas: mesenchymal stem cells (MSC) versus cancer stem cells (CSC); genomic, phenotypic and metabolic characteristics; supporting role of MSC on tumor development; tumorigenicity in SCID mice; 3D modeling in co-cultures of cancer cells with bone cells.

More details here.